High-performance liquid chromatography is one of the common instruments in the experiment. The sensitivity of the column is an important reason to ensure the accuracy of the experiment. How can we solve the problem when the sensitivity of the column is not enough? It should be analyzed from the following aspects: 1. Insufficient sample size: the solution is to increase the sample size. 　　 2 , the sample does not flow out of the column: the mobile phase or column can be changed according to the chemical nature of the sample 　　 3 , the sample does not match the detector: adjust the wavelength according to the chemistry of the sample or change the detector 　　 4 , the detector attenuation is too much: adjust the attenuation. 　　 5 , the detector time constant is too large: the solution is to reduce the time parameter 　　 6. Detector pool window pollution: The solution is to clean the pool window. 　　 7. There are bubbles in the detection tank: the solution is exhaust. 　　 8. The measuring range of the recorder is improper: adjust the voltage range. 　　 9. The flow rate of the mobile phase is not suitable: adjust the flow rate. 10. The detector and recorder exceed the calibration curve: the solution is to check the recorder and detector and redo the calibration curve.
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Usage:
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2) Strong coloring power, excellent coverage.
3) Light permanency and weatherability.
4) 6~7 grade resistance to oxidation and light permeability, 4~5 grade resistance to basic materials.
2) Used for dyeing construction materials, such as mosaic bricks, for concrete bricks, pavement, colorful tiles, roofing tiles and man-made marble. Colors for ceramic body.
3) Used in paper industry, esp. rice paper.
4) Used for surface coloration of plastic epoxy floor, color for pitch.
5) Used as a more fade-resistant dye for plastics.
6) Used as pigment for cosmetic.
7) Used as coloring Sugar-coat for medicine tablets.
How to solve the problem when the sensitivity of the HPLC column is not enough